Bioactive peptides secreted by microorganisms like Saccharomyces cerevisiae are gaining traction in cosmetics for their antimicrobial, antioxidant, anti-inflammatory, and anti-collagenase (ACE) properties. These peptides address skin concerns such as aging, inflammation, and wound healing, making them valuable in skincare. Researchers in this study, evaluated the antimicrobial, antioxidant, and wound healing properties of peptides (2โ10 kDa) from Saccharomyces cerevisiae Ethanol-Red.
Methods
The study assessed peptide bioactivity through:
- Antimicrobial Activity: Tested against dermal pathogens (e.g., Staphylococcus aureus, Candida albicans, Streptococcus pyogenes) using broth microdilution and challenge tests.
- Collagenase Activity (ACE): Measured using a colorimetric assay.
- Antioxidant Capacity: Evaluated through spectrophotometric monitoring of nitrotetrazolium blue chloride (NBT) reduction.
- Anti-inflammatory Effects: Tumor Necrosis Factor-alpha (TNF-ฮฑ) mRNA expression was quantified in lipopolysaccharide (LPS)-stimulated fibroblasts.
- Wound Healing: Tested with human fibroblasts, keratinocytes, and endothelial cells.
Key Findings
- Antimicrobial Activity: Peptides showed broad-spectrum antimicrobial activity, with Minimum Inhibitory Concentrations (MICs) ranging from 125 ยตg/mL for Staphylococcus aureus to 1000 ยตg/mL for Candida albicans. When combined with Microcareยฎ SB, they were even more effective against Candida albicans and Aspergillus brasiliensis.
- Collagenase Inhibition: Peptides inhibited collagenase by 41.8% at 250 ยตg/mL and 94.5% at 1000 ยตg/mL, suggesting anti-aging potential.
- Antioxidant Activity: Peptides exhibited antioxidant effects, reducing oxidative stress and slowing aging by scavenging free radicals.
- Anti-inflammatory Effects: Peptides reduced TNF-ฮฑ expression in fibroblasts, demonstrating anti-inflammatory potential.
- Wound Healing and Skin Regeneration: The peptides promoted tissue regeneration and reduced fibrosis, making them suitable for wound healing applications.
Researchers in this study underscored the potential of Saccharomyces cerevisiae peptides in cosmetics, particularly as natural preservatives and bioactive ingredients for anti-aging, anti-inflammatory, and skin-repair formulations. Their multifunctionalityโranging from antimicrobial protection to antioxidant and collagenase inhibitionโpositions them as a promising solution for safer, more effective skincare products. The comprehensive evaluation of these peptides opens new doors for future cosmetic formulations aimed at meeting the growing demand for natural ingredients.
Link to the study : https://tinyurl.com/3dphnuww
