Development of a Topical Serum from the Ethanolic Extract of Halobacteria trueperi TCI66207 Residues with Anti-Inflammatory and Anti-Aging properties 

Halobacteria trueperi TCI66207, an extremophilic microorganism thriving in high-salt environments, produces extracellular polysaccharides known for their promising anti-inflammatory, antioxidant, and anti-aging properties. This study focuses on the strain H. trueperi TCI66207, isolated from the Pacific Ocean at a depth of 662 m near Hualien, and its potential to enhance skin parameters. By evaluating the effects of H. trueperi TCI66207, this research aims to develop a novel functional formulation with applications in cosmetic sectors. 

Methods 

The study involved extracting bioactive compounds from H. trueperi TCI66207 residues using ethanol, followed by cream formulation. Stability tests ensured the serum’s consistency in physical characteristics over time. The anti-inflammatory properties were assessed using in vivo skin models, with inflammation levels measured post-application of the serum. Various concentrations of the extract were tested to determine the most effective formulation while monitoring any adverse skin reactions. 

Key Findings 

  1. Efficacy of H. trueperi TCI66207 Serum in Improving Skin Parameters: 
  • Significant increases were observed in skin moisture (8.9%), brightness (2.3%), and elasticity (7.3%) over 4 weeks (p < 0.05). 
  • Mechanism of action involves exopolysaccharides (EPS) enhancing moisture retention, elasticity, and skin barrier function. 
  1. Reduction of Skin Imperfections: 
  • The serum significantly reduced skin spots (11.0%), UV spots (7.6%), skin textures (15.6%), and wrinkles (27.2%) (p < 0.05). 
  • Pore size decreased by 4.6%, and collagen content increased by 30.8% after 4 weeks (p < 0.05). 
  1. Comparison to Placebo: 
  • H. trueperi TCI66207 serum demonstrated superior results in improving skin moisture and elasticity compared to the placebo, showcasing its potential as an effective skincare ingredient. 

The H. trueperi extract-based serum exhibits potential as an anti-inflammatory topical agent, showing stability, skin compatibility, and effective inflammation reduction. Limitations include the need for longer-term stability studies and broader clinical trials on diverse skin types. Future studies could explore refining extraction methods to enhance efficacy and test synergistic effects with other bioactive compounds. 

Link: https://tinyurl.com/yd8fmxj8