Skin aging, accelerated by UV-induced reactive oxygen species (ROS), leads to wrinkles and loss of elasticity. Astaxanthin and zeaxanthin (AZ-NE), potent antioxidants, offer significant protection against ROS and have potential in anti-aging skincare. This study evaluates the stability, skin penetration, and anti-wrinkle efficacy of a radiance serum containing (AZ-NE) nanoemulsions throgh ex-vivo and in-vivo evaluations
Methods
The study involved a comprehensive approach to formulate and evaluate the radiance serum. Materials included astaxanthin, zeaxanthin, surfactants, and stabilizers. The production of astaxanthin–zeaxanthin nanoemulsions was carried out using high-pressure homogenization. Key methods included solubility determination of carotenoids, purity level analysis, serum formulation, stability testing under various conditions, ex vivo permeation tests on excised skin, and skin irritation tests. Ethical approval was obtained for in vivo assessments, which measured anti-wrinkle effectiveness through clinical trials and statistical analysis.
Key Findings
- Stability: The serum maintained physicochemical properties with a 98% retention rate over 6 months under accelerated stability conditions (40°C ± 2°C and 75% RH).
- Skin Permeation: Ex vivo studies demonstrated a permeation rate of 56.2% for astaxanthin and 52.4% for zeaxanthin after 24 hours through human epidermis.
- Irritation Testing: No significant irritation was observed during skin irritation assessments, with an irritation index score of 0.5 (considered non-irritating).
- Anti-Wrinkle Efficacy: In vivo studies showed a 30% improvement in skin elasticity and a 25% reduction in the appearance of fine lines after 8 weeks of application, based on clinical evaluations.
These nanoemulsions enhance the delivery of carotenoids, providing potent antioxidant protection against free radicals. The study sample size was limited to 15 participants, and ex vivo studies utilized python skin, which may not fully represent human skin’s complex structure. Future research should explore long-term efficacy and the potential of combining these carotenoids with other active ingredients for enhanced skincare benefits.
Link to the study: https://tinyurl.com/2svf32k8
